We can now use I2C API functions by using this handle as a reference. The generator will also create an I2C typedef handle. The configuration will follow the same parameters you have set in the Peripheral Config Tab. The generated code will therefore have I2C1_Init() function in the main.c file and also have it called. In the F407 Dev Kit we have I2C1 enabled by default. Once that is done, the code generator gives a main.c file with the I2C_Init() function configured as per the settings you mention in the Cube IDE File. The initialization process for STM32 peripherals is handled by the Cube IDE. I2C may interchangeably be used in the same context as TWI (Two Wire Interface). Differentiation is done either by address of the slave device, or using a GPIO pin to enable only a single device at a time. This protocol allows communication of a single master device with multiple slave devices.
I2C uses two wires/connection channels, the clock and data commonly referred to as SCL and SDA respectively. If you are a beginner, I would recommend you look into an STM32 Project Setup guide like this one. It’s primary target is to provide developers a concise guide about integrating peripheral modules and features into active applications. This is tutorial is not intended to be a guide for learning C language or about the STM32 platform.
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